Optimization of a Protocol for Human Serum Sample Preparation for Untargeted Metabolomics Profiling Using Bradford Assay | Zendy

Isabella Brito Savieto | Zendy; Ana Valéria Colnaghi Simionato Cantú | Zendy; Lisette Jenkins | Zendy; Alessandra Sussulini | Zendy; Ingrid Lopes Barbosa | Zendy; Gustavo Henrique Bueno Duarte | Zendy; Ronei J. Poppi | Zendy; Marcos N. Eberlin | Zendy

Open Access

Optimization of a Protocol for Human Serum Sample Preparation for Untargeted Metabolomics Profiling Using Bradford Assay

Author(s) -

Isabella Brito Savieto,

Ana Valéria Colnaghi Simionato Cantú,

Lisette Jenkins,

Alessandra Sussulini,

Ingrid Lopes Barbosa,

Gustavo Henrique Bueno Duarte,

Ronei J. Poppi,

Marcos N. Eberlin

Publication year - 2017

Publication title -

anais do congresso de iniciação científica da unicamp

Language(s) - English

Resource type - Conference proceedings

ISSN - 2447-5114

DOI - 10.19146/pibic-2017-79097

Subject(s) - profiling (computer programming) , metabolomics , chromatography , protocol (science) , sample preparation , computational biology , computer science , chemistry , biology , medicine , pathology , operating system , alternative medicine

Protein precipitation is the most commom sample preparation method applied in studies involving biological matrices, such as plasma and serum, in metabolomics. However, a poor protein precipitation step may reduce the lifetime of chromatographic columns, may cause interferences in Mass Spectrometry, and may result in limited access to sample metabolites. In this work different protein precipitation approaches were evaluated in order to assess the best protocol for untargeted metabolomics studies. For this purpose, solvents selection; sample volumes; sample/solvent ratio and temperature were evaluated by a factorial planning. Serum samples were analyzed by Bradford assay.

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