Chicken viscera protein concentrate as a source of bioactive peptides: enzymatic hydrolysis as a strategy to enhance the angiotensin I-converting enzyme (ACE) inhibitory activities

In this study, protein hydrolysates from chicken viscera were obtained using different commercial proteases and a statistical mixture design. The protein hydrolysates obtained were used as a source of bioactive peptides with angiotensin I-converting enzyme (ACE) inhibitory activities (antihypertensive activity). The results from this research showed that the chicken viscera protein concentrate in its non-hydrolyzed form (control) was able to inhibit 73.9% of the activity of the angiotensin I-converting enzyme (ACE) at 1mg/mL. On the other hand, enzymatic hydrolysis enhanced the biological properties of the proteins, increasing their ACE inhibitory activities. The maximum values of inhibition detected were 82.1, 82.5 and 83.7% using the binary mixture of Flavourzyme® 500L (50%) and Alcalase® 2.4L (50%), the ternary mixture of Flavourzyme® 500L (33.33%), Alcalase® 2.4L (33.33%) and Neutrase® 0.8L (33.33%) and Neutrase® 0.8L (100%), respectively.