Synthesis of a goniothalamin triazolic analogue

Previous studies from our group showed that goniothalamin is associated with oxidative stress and apoptosis; evidence for mitochondrial impairment has been also reported.3 An approach for elucidation of its mode of action in organelles and exogenous substances is the use of fluorescent probes for cellular imaging and the benzothiadiazole (BTD) system stands out due to reduced size and selectivity for mitochondria, an organelle closely involved with apoptosis. By coupling fluorescent probes to goniothalamin it would be possible to access information about goniothalamin mode of action and click chemistry was envisaged to promote the coupling of goniothalamin to fluorescent probes through a copper (I) catalysed alkyne-azide coupling (CuAAC).