Electrochemical sensing and fluorescence imaging of E. coli O157:H7 based on aptamer-conjugated semiconducting nanoparticles | Zendy

Kadri Güleç | Zendy; Dilek ODACI DEMIRKOL | Zendy

Open Access

Electrochemical sensing and fluorescence imaging of E. coli O157:H7 based on aptamer-conjugated semiconducting nanoparticles

Author(s) -

Kadri Güleç,

Dilek ODACI DEMIRKOL

Publication year - 2018

Publication title -

süleyman demirel üniversitesi fen bilimleri enstitüsü dergisi

Language(s) - English

Resource type - Journals

eISSN - 1308-6529

pISSN - 1300-7688

DOI - 10.19113/sdufbed.11747

Subject(s) - aptamer , conjugated system , monolayer , fluorescence , quantum dot , fluorescence microscope , electrode , colloidal gold , nanoparticle , cysteamine , electrochemistry , materials science , molecule , scanning electron microscope , amine gas treating , nanotechnology , chemistry , organic chemistry , polymer , microbiology and biotechnology , optics , biology , physics , composite material

Aptamers are selective molecules against to various sizes of targets from small molecules to mammalian cells. Here, we reported QDs containing electrochemical aptasensor for the detection of E. coli O157:H7. The electrode surfaces were modified by cysteamine (CysN), which has amine and thiol groups, via self-assembled monolayer formation. The carboxyl-functionalized quantum dots (QD) and aptamers (Apt) were conjugated to cysteamine modified gold electrodes. Linear range for E. coli O157:H7 was from 1 to 10 2 CFU/mL after incubation on CysN/QD/Apt modified Au surfaces. QDs provide fluorescence surface, so that adhesion of cells was followed using fluorescence microscope. Adhered cells were also imaged by scanning electron microscopy. Finally, cell analysis was carried out in real samples.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research