Chemical Castration Using Iron (III) Chloride Hexahydrate (KEBIRI KIMIAWI MENGGUNAKAN FERIKLORIDA HEKSAHIDRAT)

Chemical castration is a method that can be applied easily without any surgical intervention in animals. This study utilized iron (III) chloride hexahydrate (FeCl 3 .6H 2 O) as a new material for chemical castration in mice. Twenty seven adult male mice were divided into five groups: FeCl 3 20% (n = 6), FeCl 3 10% (n = 6), FeCl 3 5.0% (n = 6), FeCl 3 2.5% (n = 6), and control NaCl 0.9% (n = 3). A 0.2 mL of NaCl 0.9% or FeCl 3 in various concentrations was injected intra-testicularly on each testis of the mice. Post-castration survival rate with LD 50 values was obtained at the concentrations between 2.5-5.0% of FeCl 3 groups, and 100% mice survived in the control group. The size of testis and concentration of spermatozoa decreased, in contrast with the increased concentration of FeCl 3 solution used seven days post-injection compared to the control group.