Gene Mapping of Fertility Restorer Gene(s) in Rice using STS and SSR Markers

Hybrid technology is very successful in increasing rice yield. Genetic map of fertility restorer genes using STS markers linked to fertility gene in F2 population from parental plants (IR58025A) and Amol-2 in rice was studied. Present work was conducted in biotechnology laboratory of Sari Agricultural Sciences and Natural Resources University in 2010. In current study 20 SSR markers and two STS marker were used. RG140, RM49, RM243, RM23, RM302, RM580, RM244, RM311, RM171 and RM228 showed polymorphism, while, RM128, RM104, RM258, RM84, RM269, RM3510, RM271, RM294, S10019 did not showed polymorphism. RG140, RM490, RM243 and RM23 by using Join Map Software have been grouped in one linkage group and RM244, RM311 in another group The fertility gene was mapped between RG140 and RM490 with 1 cM distance to RG140 and the distance of fertility gene to RM490 was obtained 15 cM and the other gene (Rf4) was mapped between RM244, RM311 with 7 cM distance to RM244.