Antioxidant effects of alpha-tocopherol (vitamine E) on testis regeneration in busulfan-treated mice

Background: Busulfan is a chemotherapy medicine for treatment of cancer. This medicine has cytotoxic effects on different organs of animal. Since the use of chemotherapy medicine are increasing due to individuals get involved to cancer, thus preserving of reproductive system in these patients is essential to continue fertility. Materials and Method: In present study the testis of mice were injected with different doses of busulfan (35, 40 mg/kg body weight intraperitonealy. The effects of busulfan was examinned on destruction of testis tissue, semen parameters and catalase enzymatic activity compared with control group after 30 days. Finally, fter determining the effective dose of busulfan, Vitamin E was injected to the mice as an antioxidant enzyme and its effects on semen parameters and testis tissue was examined after one month. Results: The results showed that oxidative stress was increased in busulfan-treated mice during 30 days. Catalase enzymatic activity decreased significantly in testis of Busulfan-treated mice in comparison with control group. A significant decrease of sperm number was observed according to treatment by Busulfan. After finding the most damaging dose of busulfan (40 mg/kg ) in destruction of testis tissue, these mice were injected by vitamine E. The results showed that the oxidative stress and percentage of abnormal sperm were decreased in Busulfan-treated mice that exposed a dose of 100 mg/kg vitamin E. The number of sperm and antioxidant activity of catalase was increased. Conclusion: Vitamin E improves a revival of spermatogenesis and improves testicular parameters in these patients.