A long noncoding RNA AB073614 promotes tumorigenesis and predicts poor prognosis in ovarian cancer | Zendy

Zhongping Cheng | Zendy; Jing Guo | Zendy; Li Chen | Zendy; Ning Luo | Zendy; Weihong Yang | Zendy; Xiaoyan Qu | Zendy

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A long noncoding RNA AB073614 promotes tumorigenesis and predicts poor prognosis in ovarian cancer

Author(s) -

Zhongping Cheng,

Jing Guo,

Li Chen,

Ning Luo,

Weihong Yang,

Xiaoyan Qu

Publication year - 2015

Publication title -

oncotarget

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.373

H-Index - 127

ISSN - 1949-2553

DOI - 10.18632/oncotarget.4541

Subject(s) - gene knockdown , oncogene , carcinogenesis , long non coding rna , cancer research , cell growth , biology , cell cycle , apoptosis , transfection , ovarian cancer , cell , protein kinase b , western blot , downregulation and upregulation , cell culture , cancer , microbiology and biotechnology , gene , genetics

Long noncoding RNA (lncRNA) profiles in ovarian cancer (OC) remain largely unknown. In the present study, we screened AB073614 as a new candidate lncRNA which promotes development of OC, in two independent datasets (GSE18521 and GSE38666) from the Gene Expression Omnibus (GEO). The level of AB073614 was then detected in 75 paired OC tissues and adjacent normal tissues by qRT-PCR. Results showed that AB073614 expression was significantly up-regulated in 85.3% (64/75) cancerous tissues compared with normal counterparts (P < 0.01). Further, the 5-year overall survival (OS) in OC patients with high expression of AB073614 was inferior to that with low expression (17.2 months vs 30.0 months, P = 0.0025). To investigate the functional role of AB073614, AB073614 siRNA was transfected into OC cell lines. Knockdown of AB073614 expression significantly inhibited cell proliferation and invasion, resulted in cell arrest in G1 phase of cell cycle and a dramatic increase of apoptosis, both in HO-8910 and OVCAR3 cells. In vivo experiment also revealed that knockdown AB073614 inhibited OVCAR3 cells proliferation. Finally, western blot assays indicated that lncRNA AB073614 may exert its function by targeting ERK1/2 and AKT-mediated signaling pathway. In conclusion, our study suggests that lncRNA AB073614 acts as a functional oncogene in OC development.

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