Quantitative mass spectrometry analysis reveals a panel of nine proteins as diagnostic markers for colon adenocarcinomas | Zendy

Apurva Atak | Zendy; S. K. KHURANA | Zendy; Kishore Gollapalli | Zendy; Panga Jaipal Reddy | Zendy; Roei Levy | Zendy; Stav Ben-Salmon | Zendy; Dror Hollander | Zendy; Maya Donyo | Zendy; Anke Heit | Zendy; Agnes HotzWagenblatt | Zendy; Hadas Biran | Zendy; Roded Sharan | Zendy; Shailendra Rane | Zendy; Ashutosh Shelar | Zendy; Gil Ast | Zendy; Sanjeeva Srivastava | Zendy

Open Access

Quantitative mass spectrometry analysis reveals a panel of nine proteins as diagnostic markers for colon adenocarcinomas

Author(s) -

Apurva Atak,

S. K. KHURANA,

Kishore Gollapalli,

Panga Jaipal Reddy,

Roei Levy,

Stav Ben-Salmon,

Dror Hollander,

Maya Donyo,

Anke Heit,

Agnes HotzWagenblatt,

Hadas Biran,

Roded Sharan,

Shailendra Rane,

Ashutosh Shelar,

Gil Ast,

Sanjeeva Srivastava

Publication year - 2018

Publication title -

oncotarget

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.373

H-Index - 127

ISSN - 1949-2553

DOI - 10.18632/oncotarget.24418

Subject(s) - colorectal cancer , proteome , medicine , proteomics , adenocarcinoma , malignancy , colonoscopy , liquid biopsy , colorectal adenocarcinoma , pathology , biopsy , cancer research , biology , cancer , bioinformatics , gene , biochemistry

Adenocarcinomas are cancers originating from the gland forming cells of the colon and rectal lining, and are known to be the most common type of colorectal cancers. The current diagnosis strategies for colorectal cancers include biopsy, laboratory tests, and colonoscopy which are time consuming. Identification of protein biomarkers could aid in the detection of colon adenocarcinomas (CACs). In this study, tissue proteome of colon adenocarcinomas ( n = 11) was compared with the matched control specimens ( n = 11) using isobaric tags for relative and absolute quantitation (iTRAQ) based liquid chromatography-mass spectrometry (LC-MS/MS) approach. A list of 285 significantly altered proteins was identified in colon adenocarcinomas as compared to its matched controls, which are associated with growth and malignancy of the tumors. Protein interaction analysis revealed the association of altered proteins in colon adenocarcinomas with various transcription factors and their targets. A panel of nine proteins was validated using multiple reaction monitoring (MRM). Additionally, S100A9 was also validated using immunoblotting. The identified panel of proteins may serve as potential biomarkers and thereby aid in the detection of colon adenocarcinomas.

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