Metastasis-suppressingNID2, an epigenetically-silenced gene, in the pathogenesis of nasopharyngeal carcinoma and esophageal squamous cell carcinoma | Zendy

Annie Wai Yeeng Chai | Zendy; Arthur Kwok Leung Cheung | Zendy; Wei Dai | Zendy; Josephine Mun Yee Ko | Zendy; Joseph Chok Yan Ip | Zendy; Kwok Wah Chan | Zendy; Dora LaiWan Kwong | Zendy; Wai Tong Ng | Zendy; Anne Wing Mui Lee | Zendy; Roger Kai Cheong Ngan | Zendy; Chun Chung Yau | Zendy; Stewart Y. Tung | Zendy; Victor Lee | Zendy; Alfred K. Lam | Zendy; Suja Pillai | Zendy; Simon Law | Zendy; Maria Li Lung | Zendy

Open Access

Metastasis-suppressingNID2, an epigenetically-silenced gene, in the pathogenesis of nasopharyngeal carcinoma and esophageal squamous cell carcinoma

Author(s) -

Annie Wai Yeeng Chai,

Arthur Kwok Leung Cheung,

Wei Dai,

Josephine Mun Yee Ko,

Joseph Chok Yan Ip,

Kwok Wah Chan,

Dora LaiWan Kwong,

Wai Tong Ng,

Anne Wing Mui Lee,

Roger Kai Cheong Ngan,

Chun Chung Yau,

Stewart Y. Tung,

Victor Lee,

Alfred K. Lam,

Suja Pillai,

Simon Law,

Maria Li Lung

Publication year - 2016

Publication title -

oncotarget

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.373

H-Index - 127

ISSN - 1949-2553

DOI - 10.18632/oncotarget.12889

Subject(s) - cancer research , metastasis , nasopharyngeal carcinoma , dna methylation , pathogenesis , extracellular matrix , mmp2 , methylation , cell migration , biology , medicine , cell , cancer , pathology , gene expression , gene , radiation therapy , microbiology and biotechnology , biochemistry , genetics

Nidogen-2 (NID2) is a key component of the basement membrane that stabilizes the extracellular matrix (ECM) network. The aim of the study is to analyze the functional roles of NID2 in the pathogenesis of nasopharyngeal carcinoma (NPC) and esophageal squamous cell carcinoma (ESCC). We performed genome-wide methylation profiling of NPC and ESCC and validated our findings using the methylation-sensitive high-resolution melting (MS-HRM) assay. Results showed that promoter methylation of NID2 was significantly higher in NPC and ESCC samples than in their adjacent non-cancer counterparts. Consistently, down-regulation of NID2 was observed in the clinical samples and cell lines of both NPC and ESCC. Re-expression of NID2 suppresses clonogenic survival and migration abilities of transduced NPC and ESCC cells. We showed that NID2 significantly inhibits liver metastasis. Mechanistic studies of signaling pathways also confirm that NID2 suppresses the EGFR/Akt and integrin/FAK/PLCγ metastasis-related pathways. This study provides novel insights into the crucial tumor metastasis suppression roles of NID2 in cancers.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research