Multiwalled carbon nanotubes co-delivering sorafenib and epidermal growth factor receptor siRNA enhanced tumor-suppressing effect on liver cancer | Zendy

Zhili Wen | Zendy; Yuliang Feng | Zendy; Youwen Hu | Zendy; Lingyan Lian | Zendy; Hongyan Huang | Zendy; Li Guo | Zendy; Shan-Wen Chen | Zendy; Qian Yang | Zendy; Moran Zhang | Zendy; Lijun Wan | Zendy; Kedong Xu | Zendy; Degejirifu | Zendy; Xiaohua Yan | Zendy

Open Access

Multiwalled carbon nanotubes co-delivering sorafenib and epidermal growth factor receptor siRNA enhanced tumor-suppressing effect on liver cancer

Author(s) -

Zhili Wen,

Yuliang Feng,

Youwen Hu,

Lingyan Lian,

Hongyan Huang,

Li Guo,

Shan-Wen Chen,

Qian Yang,

Moran Zhang,

Lijun Wan,

Kedong Xu,

Degejirifu,

Xiaohua Yan

Publication year - 2021

Publication title -

aging

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.473

H-Index - 90

ISSN - 1945-4589

DOI - 10.18632/aging.103905

Subject(s) - sorafenib , epidermal growth factor receptor , cancer research , chemistry , liver cancer , receptor , materials science , hepatocellular carcinoma , medicine , biochemistry

Objective: This study aimed to investigate the effects of multiwalled carbon nanotubes (MWNTs) co-delivering sorafenib (Sor) and epidermal growth factor receptor (EGFR) siRNA (MWNT/Sor/siRNA) on tumor growth in liver cancer (LC). Results: MWNT/Sor/siRNA was proved to possess increased Sor release, high siRNA stability, and enhanced cellular uptake. In addition, MWNT treatment has few effects on cell proliferation and apoptosis in HepG2 cells; however, MWNT/Sor/siRNA treatment significantly inhibited clone number and induced cell apoptosis, which shows a more favorable antitumor effect than MWNT/Sor and free Sor and free siRNA in HepG2 cells. Moreover MWNT/Sor/siRNA treatment has the most significant antitumor effect in vivo . Conclusions: MWNT/Sor/siRNA exhibited a superior antitumor effect in vitro and in vivo . Methods: The MWNT/Sor and MWNT/Sor/siRNA were prepared, and then the morphologies of MWNT/Sor/siRNA were analyzed. In vitro Sor release assay, siRNA stability and cellular uptake of MWNT/Sor/siRNA were performed as well. Next, the effects of MWNT, free Sor, free siRNA, MWNT/Sor and MWNT/Sor/siRNA were evaluated by colony-forming assay, and cell apoptosis assay in HepG2 cells. Meanwhile, the level of EGFR and proteins associated with apoptosis was tested. Furthermore, the anti-tumor effects of MWNT/Sor/siRNA on LC xenograft mice were also unraveled.

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