Open AccessT cell stimulation and expansion by SunTag-based clustering of anti-CD3/CD28 scFv
Author(s) -
Kunhong Zhong,
Zhiyong Liu,
Hongjian Li,
Shasha Zhao,
Yuelong Wang,
Wenhao Guo,
Xi Zheng,
Hui Yang,
Gang Guo,
Liangxue Zhou,
Jianguo Xu,
Aiping Tong
Publication year - 2020
Publication title -
aging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.473
H-Index - 90
ISSN - 1945-4589
DOI - 10.18632/aging.103318
Subject(s) - cd28 , cd3 , t cell , stimulation , t cell receptor , chimeric antigen receptor , antibody , microbiology and biotechnology , chemistry , antigen , cancer research , cytotoxic t cell , in vitro , biology , immunology , biochemistry , cd8 , endocrinology , immune system
Therapeutic ex vivo T cell expansion is limited by low rates and poor functionality, especially for T cells from aged cancer patients. Here, we describe a novel method for T cell stimulation and expansion using a system named SunTag-based clustering of anti-CD3/CD28 scFv (SBCS). In this method, SunTag was used to recruit up to 13 copies of anti-CD3/CD28 scFv for T cell activation. Compared with the traditional method using immobilized CD3/CD28 antibodies, the SBCS system produced approximately 1.5-fold greater expansion of T cells from healthy donors, and more than 2-fold greater expansion of T cells from aged cancer patients after stimulation. The efficiency of expansion depended mainly on the concentration of the clustered polymers of anti-CD3 scFv rather than anti-CD28 scFv. We also demonstrated that the SBCS-expanded T cells could be used to prepare functional chimeric antigen receptor modified T cells for antitumor therapy.
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