Long non-coding RNA TPT1-AS1 promotes angiogenesis and metastasis of colorectal cancer through TPT1-AS1/NF90/VEGFA signaling pathway | Zendy

Yiyun Zhang | Zendy; Jiangyun Sun | Zendy; Yuan Qi | Zendy; Yimin Wang | Zendy; Yu Ding | Zendy; Kun Wang | Zendy; Qingxin Zhou | Zendy; Jingxuan Wang | Zendy; Fei Ma | Zendy; JianGuo Zhang | Zendy; Baoliang Guo | Zendy

Open Access

Long non-coding RNA TPT1-AS1 promotes angiogenesis and metastasis of colorectal cancer through TPT1-AS1/NF90/VEGFA signaling pathway

Author(s) -

Yiyun Zhang,

Jiangyun Sun,

Yuan Qi,

Yimin Wang,

Yu Ding,

Kun Wang,

Qingxin Zhou,

Jingxuan Wang,

Fei Ma,

JianGuo Zhang,

Baoliang Guo

Publication year - 2020

Publication title -

aging

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.473

H-Index - 90

ISSN - 1945-4589

DOI - 10.18632/aging.103016

Subject(s) - angiogenesis , downregulation and upregulation , metastasis , cancer research , colorectal cancer , vascular endothelial growth factor a , messenger rna , long non coding rna , biology , cancer , gene , vegf receptors , vascular endothelial growth factor , genetics

LncRNAs have been proven closely correlated to tumor progression. A recent study identified LncRNA TPT1-AS1 (TPT1-AS1) as one of the liver-metastasis associated LncRNAs in colorectal cancer (CRC). In this study, we report that TPT1-AS1 is upregulated in CRC tissues, which is associated with poor prognosis. Functional assays unravel a pro-angiogenesis and metastasis role of TPT1-AS1. Mechanistically, Flexmap 3D assays reveal that TPT1-AS1 upregulates the VEGFA secretion in CRC cells. RNA immunoprecipitation and mRNA stability assays further show that TPT1-AS1 interacts with nuclear factor 90 (NF90) and subsequently promotes the association between NF90 and VEGFA mRNA, which leads to the upregulation of VEGFA mRNA stability. Therefore, we elucidate a new regulatory mechanism of TPT1-AS1 in CRC angiogenesis and targeting the TPT1-AS1/NF90/VEGFA axis may provide a useful strategy for diagnosis and treatment for colorectal cancer patients.

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