LncRNA MIR22HG abrogation inhibits proliferation and induces apoptosis in esophageal adenocarcinoma cells via activation of the STAT3/c-Myc/FAK signaling | Zendy

Wenmei Su | Zendy; Chunfang Guo | Zendy; Lihui Wang | Zendy; Zhuwen Wang | Zendy; Xia Yang | Zendy; Feiyu Niu | Zendy; Daniel Tzou | Zendy; Xiao Yang | Zendy; Xiaobi Huang | Zendy; Jiancong Wu | Zendy; Xiaorao Chen | Zendy; Lei Zou | Zendy; Zhixiong Yang | Zendy; Guoan Chen | Zendy

Open Access

LncRNA MIR22HG abrogation inhibits proliferation and induces apoptosis in esophageal adenocarcinoma cells via activation of the STAT3/c-Myc/FAK signaling

Author(s) -

Wenmei Su,

Chunfang Guo,

Lihui Wang,

Zhuwen Wang,

Xia Yang,

Feiyu Niu,

Daniel Tzou,

Xiao Yang,

Xiaobi Huang,

Jiancong Wu,

Xiaorao Chen,

Lei Zou,

Zhixiong Yang,

Guoan Chen

Publication year - 2019

Publication title -

aging

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.473

H-Index - 90

ISSN - 1945-4589

DOI - 10.18632/aging.102071

Subject(s) - gene silencing , cell growth , cancer research , apoptosis , western blot , cell migration , cell culture , cell , stat3 , signal transduction , biology , microbiology and biotechnology , gene , genetics

Long non-coding RNAs (lncRNAs) have involved in human malignancies and played an important role in gene regulations. The dysregulation of lncRNA MIR22HG has been reported in several cancers. However, the role of MIR22HG in esophageal adenocarcinoma (EAC) is poorly understood. Loss of function approaches were used to investigate the biological role of MIR22HG in EAC cells. The effects of MIR22HG on cell proliferation were evaluated by WST-1 and colony formation assays. The effects of MIR22HG on cell migration and invasion were examined using transwell assays. QRT-PCR and Western blot were used to evaluate the mRNA and protein expression of related genes. In this study, abrogation of MIR22HG inhibited cell proliferation, colony formation, invasion and migration in EAC 3 cell lines (OE33, OE19 and FLO-1). Mechanistically, MIR22HG silencing decreased the expression of STAT3/c-Myc/p-FAK proteins and induced apoptosis in EAC cell lines. These results delineate a novel mechanism of MIR22HG in EAC, and may provide potential targets by developing lncRNA-based therapies for EAC.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research