Development a hydrolysis probe-based quantitative PCR assay for the specific detection and quantification of Candida auris | Zendy

Hadis Jafarian | Zendy; Hossein Khodadadi | Zendy; Parisa Badiee | Zendy

Open Access

Development a hydrolysis probe-based quantitative PCR assay for the specific detection and quantification of Candida auris

Author(s) -

Hadis Jafarian,

Hossein Khodadadi,

Parisa Badiee

Publication year - 2020

Publication title -

current medical mycology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.369

H-Index - 6

eISSN - 2423-3439

pISSN - 2423-3420

DOI - 10.18502/cmm.6.3.4665

Subject(s) - candida auris , chromatography , microbiology and biotechnology , chemistry , biology , antifungal

Candida auris is an emerging multidrug-resistant pathogen. The identification of this species with the conventional phenotypic or biochemical mycological methods may lead to misidentification. Molecular-based species-specific identification methods such as quantitative real-time polymerase chain reaction (qPCR) facilitate a more reliable identification of C. auris than mycological methods. Regarding this, the present study aimed to develop a hydrolysis probe-based qPCR assay for the rapid, accurate identification of C. auris .

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