MiR-149 attenuates the proliferation and migration of TGF-β1-induced airway smooth muscle cells by targeting TRPM7 and affecting downstream MAPK signal pathway | Zendy

Zhengyu Zhu | Zendy; Liya Zhang | Zendy; Ting Jiang | Zendy; Qian Yan | Zendy; Yun Sun | Zendy; Qian Zhang | Zendy

Open Access

MiR-149 attenuates the proliferation and migration of TGF-β1-induced airway smooth muscle cells by targeting TRPM7 and affecting downstream MAPK signal pathway

Author(s) -

Zhengyu Zhu,

Liya Zhang,

Ting Jiang,

Qian Yan,

Yun Sun,

Qian Zhang

Publication year - 2020

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.2020_5417

Subject(s) - trpm7 , cell growth , mapk/erk pathway , microbiology and biotechnology , signal transduction , microrna , transforming growth factor , cell migration , cancer research , transforming growth factor beta , p38 mitogen activated protein kinases , biology , cell , receptor , medicine , transient receptor potential channel , gene , genetics , biochemistry

Asthma is considered as a general term for various chronic inflammatory diseases of the respiratory tract. Growing evidences have supported that microRNAs were involved in mediating cell proliferation, migration, and other cellular functions. MiR-149 has been found to take part in the development of various cancers. However, whether miR-149 participated in the proliferation and migration of transforming growth factor beta 1 (TGF-β1)-induced airway smooth muscle cells was still unknown. In this study, the expression level of miR-149 in human airway smooth muscle cells (ASMCs) was decreased after TGF-β1 treatment in vitro. Additionally, the over-expression of miR-149 obviously suppressed proliferation and migration in human ASMCs. Besides, we found that overexpression of miR-149 could inhibit the expression of transient receptor potential melastatin 7 (TRPM7) both in protein and gene levels. Furthermore, we demonstrated that miR-149 could inhibit the cell proliferation and migration in human ASMCs by targeting TRPM7 through modulating mitogen-activated protein kinases (MAPKs) signaling pathway. Taken together, we strongly supported that miR-149 might be a key inhibitor of asthma by targeting TRMP7. Therefore, our finding suggests a promising biomarker for the development of further targeted therapies for asthma.

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