Potential limitations of the Sleeping Beauty transposon use in gene expression studies | Zendy

Weronika Sowińska | Zendy; Mateusz Wawro | Zendy; Aleksandra Solecka | Zendy; Aneta Kasza | Zendy

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Potential limitations of the Sleeping Beauty transposon use in gene expression studies

Author(s) -

Weronika Sowińska,

Mateusz Wawro,

Aleksandra Solecka,

Aneta Kasza

Publication year - 2019

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.2019_2839

Subject(s) - sleeping beauty transposon system , transposable element , gene , beauty , gene expression , biology , transgene , genetics , rnase p , microbiology and biotechnology , rna , genome , philosophy , epistemology

MCPIP2 is the least known member of the MCPIP family of proteins. Recently we have found that it is a new RNase involved in transcript turnover. However, the full spectrum of its cellular targets is still unidentified. To discover transcripts which are regulated by this protein we have employed Sleeping Beauty transposons. This tool allows for rapid generation of a stable transgenic cell line with inducible expression of the desired gene. In this study, we analysed how the Sleeping Beauty system itself influences expression of chosen genes, namely IL-6, Regnase-1 and VEGF. We found that the system alone may influence expression of IL-6. Our results indicate that Sleeping Beauty transposons should be used with caution in studies that are focused on changes in the transcript level.

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