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Oxalic acid degradation by a novel fungal oxalate oxidase from Abortiporus biennis
Author(s) -
Marcin Grąz,
Kamila Rachwał,
Radosław Zan,
Anna JaroszWilkołazka
Publication year - 2016
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.2016_1282
Subject(s) - oxalic acid , chemistry , oxalate , enzyme , ion chromatography , sephadex , chromatography , mycelium , organic acid , molecular mass , size exclusion chromatography , enzyme assay , biochemistry , biology , organic chemistry , botany
Oxalate oxidase was identified in mycelial extracts of a basidiomycete Abortiporus biennis strain. Intracellular enzyme activity was detected only after prior lowering of the pH value of the fungal cultures by using oxalic or hydrochloric acids. This enzyme was purified using size exclusion chromatography (Sephadex G-25) and ion-exchange chromatography (DEAE-Sepharose). This enzyme exhibited optimum activity at pH 2 when incubated at 40°C, and the optimum temperature was established at 60°C. Among the tested organic acids, this enzyme exhibited specificity only towards oxalic acid. Molecular mass was calculated as 58 kDa. The values of Km for oxalate and Vmax for the enzyme reaction were 0.015 M and 30 mmol min(-1), respectively.

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