Rhizobium strains differ considerably in outer membrane permeability and polymyxin B resistance | Zendy

Iwona Komaniecka | Zendy; Katarzyna Zamłyńska | Zendy; Radosław Zan | Zendy; Magdalena Staszczak | Zendy; Jarosław Pawelec | Zendy; Irena Seta | Zendy; Adam Choma | Zendy

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Rhizobium strains differ considerably in outer membrane permeability and polymyxin B resistance

Author(s) -

Iwona Komaniecka,

Katarzyna Zamłyńska,

Radosław Zan,

Magdalena Staszczak,

Jarosław Pawelec,

Irena Seta,

Adam Choma

Publication year - 2016

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.2015_1212

Subject(s) - rhizobia , microbiology and biotechnology , bradyrhizobium japonicum , escherichia coli , polymyxin , bacterial outer membrane , membrane permeability , biology , mesorhizobium , rhizobium , rhizobium leguminosarum , polymyxin b , bacteria , rhizobiaceae , chemistry , biochemistry , membrane , nitrogen fixation , symbiosis , gene , genetics , antibiotics

Six rhizobium (Rhizobium leguminosarum bv. Trifolii TA1, Sinorhizobium meliloti 1021, Mesorhizobium huakuii IFO 15243(T), Ochrobactrum lupini LUP 21(T), Bradyrhizobium japonicum USDA110 and B. elkanii USDA 76) and two Escherichia coli strains (E. coli ATCC 25922 and E. coli HB 101) were compared in respect to polymyxin B and EDTA resistance, as well as bacterial outer membrane (OM) permeability to a fluorescent hydrophobic agent (N-phenyl-1-naphthylamine - NPN). TEM (Transmission Electron Microscopy) and a microbial test demonstrated that all the rhizobia were much more resistant to polymyxin B in comparison with E. coli strains. EDTA and polymyxin B enhance permeability of B. japonicum and O. lupini OM. Other rhizobia incorporated NPN independently of the presence of membrane-deteriorating agents; however, the level of fluorescence (measured as NPN absorption) was strain dependent.

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