The presence of Chlamydia phage PhiCPG1 capsid protein VP1 genes and antibodies in patients infected with Chlamydia trachomatis | Zendy

Jingyue Ma | Zendy; Yuan Liu | Zendy; Yuanjun Liu | Zendy; Lingjie Li | Zendy; Shuping Hou | Zendy; Xibo Gao | Zendy; Manli Qi | Zendy; Quanzhong Liu | Zendy

Open Access

The presence of Chlamydia phage PhiCPG1 capsid protein VP1 genes and antibodies in patients infected with Chlamydia trachomatis

Author(s) -

Jingyue Ma,

Yuan Liu,

Yuanjun Liu,

Lingjie Li,

Shuping Hou,

Xibo Gao,

Manli Qi,

Quanzhong Liu

Publication year - 2016

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.2015_1185

Subject(s) - chlamydia trachomatis , capsid , chlamydia , virology , antibody , western blot , biology , chlamydiaceae , chlamydophila pneumoniae , microbiology and biotechnology , chlamydiales , immunoassay , gene , virus , immunology , genetics

Chlamydia phage PhiCPG1 has been found in Chlamydia caviae in a guinea pig model for inclusion conjunctivitis, raising the possibility that Chlamydia phage is also present in patients infected with C. trachomatis (Ct). In the present study, we assayed for presence of Chlamydia phage capsid protein VP1 genes and antibodies in 84 non-Ct controls and 206 Ct patients using an enzyme-linked immunoassay (ELISA), followed by verification with Western blot. None of the subjects were exposed to an antibiotic treatment or had a C. pneumoniae infection. The VP1 antibody test was positive in both, the ELISA and Western blot assay, in 4 Ct patients. PCR amplification experiments revealed presence of the VP1 gene in 5 Ct patients. The results suggest that Chlamydia phage capsid protein VP1 may exist in some Ct patients.

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