Global quantification of heterochromatin-associated histone methylations in cell lines with differential sensitivity to ionizing radiation | Zendy

Merve Cetinkaya | Zendy; Emre Özgür | Zendy; Nejat Dalay | Zendy; Uğur Gezer | Zendy

Open Access

Global quantification of heterochromatin-associated histone methylations in cell lines with differential sensitivity to ionizing radiation

Author(s) -

Merve Cetinkaya,

Emre Özgür,

Nejat Dalay,

Uğur Gezer

Publication year - 2015

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.2014_790

Subject(s) - hela , histone , microbiology and biotechnology , dna methylation , heterochromatin , dna damage , ionizing radiation , chemistry , dna , cell culture , chromatin , biology , cell , irradiation , genetics , gene expression , biochemistry , gene , physics , nuclear physics

Histone modifications are involved in the DNA damage response (DDR). Here, by utilizing an ELISA immunoassay we assessed the methylation at H3K9 (H3K9me2 and H3K9me3) in two cell lines with differential sensitivity to radiation-induced apoptosis, HeLa (sensitive) and MCF-7 (resistant). We found that DNA damage induction by γ-irradiation leads to considerable accumulation (up to 5-fold) of H3K9me2 and H3K9me3, but not of H4K20me3 (control modification) in MCF-7 cells (p<0.05). Interestingly, a lower dose (2 Gy) was more effective than 5 Gy. In HeLa cells a smaller effect (approx. 1.5-1.8-fold) was evident only at 5 Gy. In conclusion, our findings reveal that DNA damage leads to specific accumulation of H3K9me2 and H3K9me3 in a cell-type specific manner.

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