Differentiation by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) of Candida albicans isolated from upper respiratory tract in patients with non-small cell lung cancer. | Zendy

Anna Biernasiuk | Zendy; Izabela KoronaGłowniak | Zendy; Agnieszka Grzegorczyk | Zendy; Anna Malm | Zendy

Open Access

Differentiation by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) of Candida albicans isolated from upper respiratory tract in patients with non-small cell lung cancer.

Author(s) -

Anna Biernasiuk,

Izabela KoronaGłowniak,

Agnieszka Grzegorczyk,

Anna Malm

Publication year - 2014

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.2014_1837

Subject(s) - rapd , candida albicans , genotype , polymerase chain reaction , biology , respiratory tract , microbiology and biotechnology , lung cancer , corpus albicans , cancer , genomic dna , genetic diversity , dna , gene , respiratory system , genetics , pathology , medicine , population , environmental health , anatomy

Cancer patients are predisposed to fungal infections caused by Candida albicans, especially to oral or respiratory tract candidiasis. The aim of this study was to estimate genetic diversity by RAPD-PCR (random amplified polymorphic DNA-polymerase chain reaction) of C. albicans isolated from upper respiratory tract of 100 patients with non-small cell lung cancer. Among 52 strains, 34 genotypes were defined. 10 clusters comprising 28 (53.85%) isolates with similarity coefficient ≥ 80% were formed. The remaining 24 (46.15%) isolates represented individual genotypes. The RAPD-PCR technique revealed genomic variability within C. albicans isolated from upper respiratory tract of the cancer patients.

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