New method for the study of Amaryllidaceae alkaloid biosynthesis using biotransformation of deuterium-labeled precursor in tissue cultures. | Zendy

Anna El Tahchy | Zendy; Michel Boisbrun | Zendy; Agata Ptak | Zendy; François Dupire | Zendy; Françoise Chrétien | Zendy; Max Henry | Zendy; Yves Chapleur | Zendy; Dominique LaurainMattar | Zendy

Open Access

New method for the study of Amaryllidaceae alkaloid biosynthesis using biotransformation of deuterium-labeled precursor in tissue cultures.

Author(s) -

Anna El Tahchy,

Michel Boisbrun,

Agata Ptak,

François Dupire,

Françoise Chrétien,

Max Henry,

Yves Chapleur,

Dominique LaurainMattar

Publication year - 2010

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.2010_2375

Subject(s) - lycorine , amaryllidaceae alkaloids , alkaloid , biotransformation , amaryllidaceae , chemistry , biosynthesis , deuterium , stereochemistry , phenol , chromatography , mass spectrometry , biochemistry , organic chemistry , botany , biology , physics , quantum mechanics , enzyme

Biotransformation of deuterated-4'-O-methylnorbelladine into alkaloids galanthamine and lycorine in tissue cultures of Leucojum aestivum was demonstrated using HPLC coupled to mass spectrometry. GC-MS screening was also carried to investigate other native and deuterated alkaloids. A total of six labeled alkaloids were identified indicating that 4'-O-methyl-d(3)-norbelladine is incorporated into three different groups of Amaryllidaceae alkaloids that are biosynthesized by three modes of intramolecular oxidative phenol coupling.

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