Reference genes for gene expression studies on non-small cell lung cancer. | Zendy

Peter Grešner | Zendy; Jolanta Gromadzińska | Zendy; Wojciech Wąsowicz | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Reference genes for gene expression studies on non-small cell lung cancer.

Author(s) -

Peter Grešner,

Jolanta Gromadzińska,

Wojciech Wąsowicz

Publication year - 2009

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.2009_2463

Subject(s) - reference genes , gene , database normalization , gene expression , normalization (sociology) , biology , lung cancer , housekeeping gene , gene expression profiling , real time polymerase chain reaction , glyceraldehyde 3 phosphate dehydrogenase , computational biology , cell , microbiology and biotechnology , genetics , pathology , medicine , computer science , cluster analysis , machine learning , sociology , anthropology

STUDY OBJECTIVEThe aim of this study was to test a panel of 6 reference genes in order to identify and validate the most suitable reference genes for expression studies in paired healthy and non-small cell lung cancer tissues.METHODQuantitative real-time PCR followed by the NormFinder- and geNorm-based analysis was employed. The study involved 21 non-small cell lung cancer patients.RESULTSThe analysis of experimental data revealed HPRT1 as the most stable gene followed by RPLP0 and ESD. In contrast, GAPDH was found to be the least stable gene. HPRT1 together with ESD was revealed as the pair of genes introducing the least systematic error into data normalization. Validation by bootstrap random sampling technique and by normalizing exemplary gene expression data confirmed the results.CONCLUSIONAlthough HPRT1 and ESD may by recommended for data normalization in gene expression studies on non-small cell lung cancer, the suitability of selected reference genes must be unconditionally validated prior to each study.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research