The nuclear cap-binding protein complex is not essential for nonsense-mediated mRNA decay (NMD) in plants. | Zendy

Agnieszka DzikiewiczKrawczyk | Zendy; Paulina Piontek | Zendy; Zofia SzweykowskaKulinska | Zendy; Artur Jarmołowski | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

The nuclear cap-binding protein complex is not essential for nonsense-mediated mRNA decay (NMD) in plants.

Author(s) -

Agnieszka DzikiewiczKrawczyk,

Paulina Piontek,

Zofia SzweykowskaKulinska,

Artur Jarmołowski

Publication year - 2008

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.2008_3047

Subject(s) - nonsense mediated decay , mutant , arabidopsis thaliana , gene , messenger rna , biology , microbiology and biotechnology , wild type , splice , genetics , rna splicing , rna

In this study we investigated whether in plants, like in mammals, components of the nuclear cap-binding protein complex (CBC) are involved in nonsense-mediated mRNA decay (NMD). We selected several genes producing at least two alternatively spliced mRNA variants: one with a premature termination codon (PTC+) and another without it (PTC-). For each gene the PTC+/PTC- ratio was calculated using RT-PCR and direct sequencing in four Arabidopsis thaliana lines: wild type, the NMD mutant atupf3-1 and two CBC mutants: cbp20 and abh1. Whereas in the NMD mutant the ratios of PTC+/PTC- splice variants were higher than in wild-type plants, the two CBC mutants investigated showed no change in the PTC+/PTC- ratios. Our results suggest that neither CBP20 nor CBP80 is involved in NMD in A. thaliana.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research