SNP-minisequencing as an excellent tool for analysing degraded DNA recovered from archival tissues. | Zendy

Katarzyna BąbolPokora | Zendy; Jarosław Berent | Zendy

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SNP-minisequencing as an excellent tool for analysing degraded DNA recovered from archival tissues.

Author(s) -

Katarzyna BąbolPokora,

Jarosław Berent

Publication year - 2008

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.2008_3045

Subject(s) - biology , multiplex , snp , polymerase chain reaction , dna , genetics , multiplex polymerase chain reaction , single nucleotide polymorphism , microbiology and biotechnology , gene , genotype

SNP-minisequencing has become common in forensic genetics, especially for analysing degraded or low copy number DNA (LCN DNA). The aim of this study was to examine the usefulness of five SNP (single nucleotide polymorphism) markers for analyzing degraded and LCN DNA recovered from archival samples. DNA extractions of eight formalin-fixed paraffin-embedded (FFPE) tissues were performed and DNA fragments were amplified in one multiplex PCR (polymerase chain reaction). SNPs were identified in a minisequencing reaction and a gel electrophoresis in ABI Prism 377 Sequencer. The research confirmed the usefulness of SNP-minisequencing for analysing FFPE tissues.

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