Molecular cloning and expression analysis of a new gene for short-chain dehydrogenase/reductase 9. | Zendy

Shen Liu | Zendy; Chaoqun Huang | Zendy; Dawei Li | Zendy; Weihua Ren | Zendy; Haoxing Zhang | Zendy; Qi Meiyan | Zendy; Xin Li | Zendy; Long Yu | Zendy

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Molecular cloning and expression analysis of a new gene for short-chain dehydrogenase/reductase 9.

Author(s) -

Shen Liu,

Chaoqun Huang,

Dawei Li,

Weihua Ren,

Haoxing Zhang,

Qi Meiyan,

Xin Li,

Long Yu

Publication year - 2007

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.2007_3289

Subject(s) - complementary dna , gene , cloning (programming) , microbiology and biotechnology , molecular cloning , biology , expression vector , escherichia coli , cdna library , cloning vector , signal peptide , recombinant dna , gene expression , genomic library , reductase , peptide sequence , genetics , biochemistry , enzyme , computer science , programming language

We report here the cloning and characterization of a novel human short-chain dehydrogenases/reductase gene SCDR9, isolated from a human liver cDNA library, and mapped to 4q22.1 by browsing the UCSC genomic database. SCDR9 containing an ORF with a length of 900 bp, encoding a protein with a signal peptide sequence and an adh_short domain. GFP localization shows SCDR9 protein concentrated in some site of the cytoplasm, but not in the ER. Expression pattern in eighteen tissues revealed that SCDR9 is expressed highly in liver. Soluble recombinant protein was successfully purified from Escherichia coli using pET28A(+) expression vector. Our data provides important information for further study of the function of the SCDR9 gene and its products.

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