Up-regulation of human PNPase mRNA by beta-interferon has no effect on protein level in melanoma cell lines. | Zendy

Kamil Gewartowski | Zendy; Rafał Tomecki | Zendy; Lukasz Muchowski | Zendy; Aleksandra Dmochow Ska | Zendy; Artur Dzwonek | Zendy; Michał Małecki | Zendy; H Skurzak | Zendy; Jerzy Ostrowski | Zendy; Piotr P. Stȩpień | Zendy

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Up-regulation of human PNPase mRNA by beta-interferon has no effect on protein level in melanoma cell lines.

Author(s) -

Kamil Gewartowski,

Rafał Tomecki,

Lukasz Muchowski,

Aleksandra Dmochow Ska,

Artur Dzwonek,

Michał Małecki,

H Skurzak,

Jerzy Ostrowski,

Piotr P. Stȩpień

Publication year - 2006

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.2006_3376

Subject(s) - polynucleotide phosphorylase , messenger rna , hela , melanoma , exoribonuclease , interferon , jurkat cells , mitochondrion , microbiology and biotechnology , purine nucleoside phosphorylase , cell culture , protein biosynthesis , cell , biology , chemistry , cancer research , enzyme , rna , immunology , gene , rnase p , biochemistry , t cell , genetics , immune system , purine

Human mitochondrial polynucleotide phosphorylase (hPNPase) is an exoribonuclease localized in mitochondria. The exact physiological function of this enzyme is unknown. Recent studies have revealed the existence of a relationship between induction of hPNPase mRNA and both cellular senescence and growth arrest of melanoma cells following beta-interferon treatment. The aim of this study was to verify whether the augmented hPNPase mRNA level results in increase of the protein level. In several cell lines established from five metastatic melanoma patients we did not find any such correlation. However, an elevated level of hPNPase protein was observed in interferon-induced HeLa and Jurkat cells. This increase was correlated with a slight shortening of poly(A) tails of mitochondrial ND3 transcript.

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