Mapping of a transcription promoter located inside the priA gene of the Bacillus subtilis chromosome. | Zendy

Krzysztof Hinc | Zendy; Adam Iwanicki | Zendy; Simone J. Séror | Zendy; Michał Obuchowski | Zendy

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Mapping of a transcription promoter located inside the priA gene of the Bacillus subtilis chromosome.

Author(s) -

Krzysztof Hinc,

Adam Iwanicki,

Simone J. Séror,

Michał Obuchowski

Publication year - 2006

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.2006_3320

Subject(s) - gene , transcription (linguistics) , biology , bacillus subtilis , rna polymerase , promoter , sigma factor , genetics , housekeeping gene , microbiology and biotechnology , rna , gene expression , bacteria , philosophy , linguistics

The genome sequence of the Gram-positive soil bacterium Bacillus subtilis was completed in 1997 (Kunst et al., 1998) and the results included the identification of a putative transcription unit encompassing the yloI to yloS genes. Within this region of the B. subtilis chromosome 11 putative open reading frames were found with a wide diversity of probable functions. In this work we have analyzed transcription in the region of the priA-cpgA genes and we have mapped a promoter which is located inside the priA gene and its activity directs transcription of the def-yloM genes. Moreover, this transcript can be extended at low level to the prpC-priK-cpgA genes. Analysis of the sequence in proximity of the transcription start site revealed a sequence suitable for the housekeeping sigma(A) subunit of RNA polymerase. Analysis of the beta-glactosidase activity of transcription fusions revealed that the identified promoter is active at low level and its activity is increased during late exponential phase of growth.

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