Mitochondrial transcription factor A is the major protein in rodent hepatocytes that recognizes DNA lesions induced by N-acetoxy-acetylaminofluorene. | Zendy

Monika Pietrowska | Zendy; Izabela Kołodziejczyk | Zendy; Piotr Widłak | Zendy

Open Access

Mitochondrial transcription factor A is the major protein in rodent hepatocytes that recognizes DNA lesions induced by N-acetoxy-acetylaminofluorene.

Author(s) -

Monika Pietrowska,

Izabela Kołodziejczyk,

Piotr Widłak

Publication year - 2006

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.2006_3306

Subject(s) - tfam , dna , microbiology and biotechnology , benzo(a)pyrene , mitochondrial dna , 2 acetylaminofluorene , chemistry , recombinant dna , dna damage , carcinogen , transcription (linguistics) , transcription factor , nuclear dna , dna repair , biology , biochemistry , gene , enzyme , linguistics , philosophy , microsome

Extracts from rodent liver cells contain an abundant protein that recognizes DNA adducts induced by the chemical carcinogen N-acetoxy-acetylaminofluorene (AAAF). This protein also has a strong affinity for DNA damaged by cisplatin (DDP), but not by benzo(a)pyrene diolepoxide or UV-radiation, and has been termed AAAF/DDP-DDB. Here we purified this protein from rat tissue and analyzed it by mass spectrometry and identified it as mitochondrial transcription factor A (TFAM). Experiments with bacterially expressed recombinant TFAM confirmed its high affinity for DNA damaged by AAAF. Assuming its abundance and specificity for AAAF induced lesions, TFAM may significantly impede recognition and repair of DNA adducts induced by AAAF and other derivatives of 2-aminofluorene.

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