Genetic immunization of ducks for production of antibodies specific to Helicobacter pylori UreB in egg yolks. | Zendy

Kacper Kazimierczuk | Zendy; Lucyna Cova | Zendy; Bénédicte Ndeboko | Zendy; Urszula Szczyrk | Zendy; Aneta Targosz | Zendy; Tomasz Brzozowski | Zendy; Agnieszka Sirko | Zendy

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Genetic immunization of ducks for production of antibodies specific to Helicobacter pylori UreB in egg yolks.

Author(s) -

Kacper Kazimierczuk,

Lucyna Cova,

Bénédicte Ndeboko,

Urszula Szczyrk,

Aneta Targosz,

Tomasz Brzozowski,

Agnieszka Sirko

Publication year - 2005

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.2005_3517

Subject(s) - polyclonal antibodies , helicobacter pylori , immunization , recombinant dna , antibody , titer , urease , plasmid , biology , antigen , microbiology and biotechnology , escherichia coli , gene , enzyme , immunology , genetics , biochemistry

Following genetic immunization of laying ducks with a plasmid expressing Helicobacter pylori UreB (large subunit of urease), IgY against UreB were obtained from egg yolks. These polyclonal and monospecific IgY antibodies are of higher-titer and specifically recognize recombinant H. pylori urease purified from Escherichia coli. To our knowledge this is the first report describing generation of IgY antibodies directed against antigens of H. pylori by DNA-based immunization.

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