Effect of deletion mutation on the recombination activity of Cre recombinase. | Zendy

Rongrong Liu | Zendy; Lixia Wang | Zendy; Zhongping Lin | Zendy

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Effect of deletion mutation on the recombination activity of Cre recombinase.

Author(s) -

Rongrong Liu,

Lixia Wang,

Zhongping Lin

Publication year - 2005

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.2005_3470

Subject(s) - recombinase , mutant , recombination , cre recombinase , flp frt recombination , escherichia coli , genetics , biology , site specific recombination , bacteriophage , dna , mutation , microbiology and biotechnology , cre lox recombination , tn3 transposon , genetic recombination , gene , transgene , transposable element , genetically modified mouse

Cre recombinase from bacteriophage P1 is widely used in both in vitro and in vivo DNA manipulations. Based on a structural and functional analysis, three deleted cre mutants were constructed and expressed in Escherichia coli. Mutated recombinases were purified and their recombination activities were determined in vitro. Our results revealed that the mutant with amino-terminal deletion retains the recombination activity as high as wild type Cre; however, the carboxy-terminal deletion and the middle region deletion both lead to a complete loss of the recombinase function.

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