Cellular level of 8-oxo-2'-deoxyguanosine in DNA does not correlate with urinary excretion of the modified base/nucleoside. | Zendy

Marek Foksiński | Zendy; Daniel Gackowski | Zendy; Rafał Różalski | Zendy; Ryszard Ólinski | Zendy

Open Access

Cellular level of 8-oxo-2'-deoxyguanosine in DNA does not correlate with urinary excretion of the modified base/nucleoside.

Author(s) -

Marek Foksiński,

Daniel Gackowski,

Rafał Różalski,

Ryszard Ólinski

Publication year - 2003

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.2003_3708

Subject(s) - deoxyguanosine , high performance liquid chromatography , nucleoside , chemistry , isotope dilution , dna , chromatography , 8 hydroxy 2' deoxyguanosine , urine , excretion , urinary system , dna damage , biochemistry , dna oxidation , mass spectrometry , biology , endocrinology

We assessed a relationship between the level of 8-oxodG in leukocyte DNA measured with the high performance liquid chromatography with electrochemical detection (HPLC/EC) technique and urinary excretion of the modified nucleoside/base analysed with a recently developed methodology involving HPLC prepurification followed by gas chromatography with isotope dilution mass spectrometric detection. No correlation was found between these markers of oxidative DNA damage commonly used in epidemiological studies. Several possible explanations of this finding are discussed.

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