Oligodeoxyadenylate stimulates the protein kinase activity of anti-DNA sIgA from human milk. | Zendy

Yu. Ya. Kit | Zendy; Elena V. Kuligina | Zendy; Д. Е. Семенов | Zendy; Vladimir A. Richter | Zendy

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Oligodeoxyadenylate stimulates the protein kinase activity of anti-DNA sIgA from human milk.

Author(s) -

Yu. Ya. Kit,

Elena V. Kuligina,

Д. Е. Семенов,

Vladimir A. Richter

Publication year - 2002

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.2002_3848

Subject(s) - casein kinase 2 , dna , protein kinase a , biochemistry , kinase , casein , microbiology and biotechnology , phosphorylation , chemistry , incubation , dna synthesis , biology , mitogen activated protein kinase kinase

Preparations of anti-DNA sIgA were obtained from human milk by sequential chromatography on protein A-sepharose, DEAE-fractogel and DNA-cellulose. The influence of oligonucleotides on protein kinase activity was investigated. It was discovered that incubation of anti-DNA sIgA with oligodeoxyriboadenylate d(A)12 stimulates the phosphorylation of polypeptides of sIgA in the presence of [gamma-32P]ATP. The greatest was the incorporation of P into the sIgA H-chains. We also demonstrated stimulation of the casein kinase activity of anti-DNA sIgA by d(A)12. The stimulation of the protein kinase activity of anti-DNA sIgA by oligoriboadenylate r(A)12 was not detected.

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