Cloning and characterization of the gene encoding ribosomal P0 phosphoprotein from Neurospora crassa. | Zendy

Dawid Krokowski | Zendy; Marek Tchórzewski | Zendy; Nikodem Grankowski | Zendy

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Cloning and characterization of the gene encoding ribosomal P0 phosphoprotein from Neurospora crassa.

Author(s) -

Dawid Krokowski,

Marek Tchórzewski,

Nikodem Grankowski

Publication year - 2002

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.2002_3816

Subject(s) - neurospora crassa , ribosomal protein , biology , gene , homology (biology) , complementary dna , cloning (programming) , genetics , microbiology and biotechnology , ribosomal rna , saccharomyces cerevisiae , ribosome , rna , mutant , computer science , programming language

A gene for ribosomal protein P0 that belongs to the family of ribosomal P proteins was isolated from a Neurospora crassa cDNA library, using polyclonal antibodies against recombinant P0 protein from Saccharomyces cerevisiae. This is the first gene for ribosomal P0 protein to be cloned from filamentous fungi. The derived P0 protein sequence has a strong homology to other eukaryotic P0 proteins; yet, there is a notable alteration in the conservative C-terminal region, placing this protein among the unique sequences from protozoan parasites.

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