High performance liquid chromatography and photodiode array detection of ferulic acid in Rubus protoplasts elicited by O-glycans from Fusarium sp. M7-1. | Zendy

Mihai Niță-Lazar | Zendy; Lionel Chevolot | Zendy; Shojiro Iwahara | Zendy; Kaoru Takegawa | Zendy; Aleksandra Furmanek | Zendy; Y. Liénart | Zendy

Open Access

High performance liquid chromatography and photodiode array detection of ferulic acid in Rubus protoplasts elicited by O-glycans from Fusarium sp. M7-1.

Author(s) -

Mihai Niță-Lazar,

Lionel Chevolot,

Shojiro Iwahara,

Kaoru Takegawa,

Aleksandra Furmanek,

Y. Liénart

Publication year - 2002

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.2002_3762

Subject(s) - phenylpropanoid , phenylalanine ammonia lyase , glycan , biochemistry , chemistry , inducer , phenylalanine , botany , biology , biosynthesis , glycoprotein , amino acid , enzyme , gene

So far only little data have been available concerning the eliciting capacity of well defined glycan molecules isolated from plant pathogens. This study brings new information about changes in plant cells caused by fungal pathogens. Sugar fractions derived from glycoproteins isolated from the fungus Fusarium sp. M7-1 have been tested here as signaling molecules. The ability of three O-glycan fractions (named in this work inducer I, II, III) to trigger responses in Rubus protoplasts has been examined. It was found that inducer III was the most efficient as it elicited changes in the levels of phenylpropanoid pathway intermediates in relation to phenylalanine-ammonia lyase (PAL) activation.

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