Expression of bovine leukemia virus protein p24 in Escherichia coli and its use in the immunoblotting assay. | Zendy

L. Bicka | Zendy; Jacek Kuźmak | Zendy; B. Kozaczyńska | Zendy; Andrzej Płucienniczak | Zendy; Anna Skorupska | Zendy

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Expression of bovine leukemia virus protein p24 in Escherichia coli and its use in the immunoblotting assay.

Author(s) -

L. Bicka,

Jacek Kuźmak,

B. Kozaczyńska,

Andrzej Płucienniczak,

Anna Skorupska

Publication year - 2001

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.2001_5130

Subject(s) - bovine leukemia virus , escherichia coli , fusion protein , biology , microbiology and biotechnology , plasmid , monoclonal antibody , antibody , virus , thioredoxin , virology , gene , recombinant dna , biochemistry , immunology

The gag gene encoded protein, p24 of bovine leukemia virus (BLV), was cloned and expressed as thioredoxin-6xHis-p24 protein in Escherichia coli. The bacterial cells carrying plasmid pT7THis-p24 expressed the protein of 38 kDa that was detected by immunoblotting analysis using anti-p24 monoclonal antibodies and sera from BLV infected cattle and sheep. The purified p24 fusion protein was shown to be sensitive and specific for detection of BLV antibodies in the infected cattle.

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