Direct in vivo transfer of plasmid DNA into murine tumors: effects of endotoxin presence and transgene localization. | Zendy

Magdalena Budryk | Zendy; Tomasz Cichoń | Zendy; Stanisław Szala | Zendy

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Direct in vivo transfer of plasmid DNA into murine tumors: effects of endotoxin presence and transgene localization.

Author(s) -

Magdalena Budryk,

Tomasz Cichoń,

Stanisław Szala

Publication year - 2001

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.2001_3914

Subject(s) - transgene , in vivo , transfection , plasmid , microbiology and biotechnology , dna , chemistry , genetic enhancement , biology , cancer research , gene , biochemistry , genetics

The purpose of this study was to investigate the effect of endotoxin presence in plasmid DNA preparations on the efficiency of transfection achieved in vivo with B16(F10) and Renca tumors and to determine transgene localization. Our data show that endotoxin markedly decreases the efficiency of transfection. Furthermore, the transgene transferred in vivo can be found in both neoplastic and normal (most likely myofibroblast) cells lying in proximity of the administration site.

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