Inhibition of the helicase activity of HCV NTPase/helicase by 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide-5 '-triphosphate (ribavirin-TP). | Zendy

Peter Borowski | Zendy; Melanie Lang | Zendy; Andreas Niebuhr | Zendy; Annemarie Haag | Zendy; Herbert Schmitz | Zendy; Julian Schulze zur Wiesch | Zendy; Joonho Choe | Zendy; M. A. Siwecka | Zendy; Tadeusz Kulikowski | Zendy

Open Access

Inhibition of the helicase activity of HCV NTPase/helicase by 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide-5 '-triphosphate (ribavirin-TP).

Author(s) -

Peter Borowski,

Melanie Lang,

Andreas Niebuhr,

Annemarie Haag,

Herbert Schmitz,

Julian Schulze zur Wiesch,

Joonho Choe,

M. A. Siwecka,

Tadeusz Kulikowski

Publication year - 2001

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.2001_3908

Subject(s) - helicase , ribavirin , chemistry , rna helicase a , hepatitis c virus , enzyme , carboxamide , biochemistry , virology , rna , virus , biology , gene

In the presented study the ribavirin-TP--an established inhibitor of the NTPase activity of the superfamily NTPase/helicases II--was investigated as an inhibitor of the unwinding activity of the hepatitis C virus (HCV) NTPase/helicase. The kinetics of the reaction revealed that ribavirin-TP reduces the turnover number of the helicase reaction by a mechanism that does not correspond to that of the inhibition of the NTPase activity. Our results suggest that derivatives of ribavirin-TP with enhanced stability towards hydrolytic attack may be effective inhibitors of the enzyme.

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