Electroporated intact BY-2 tobacco culture cells as a model of transient expression study. | Zendy

Edyta Kościańska | Zendy; Krzysztof Wypijewski | Zendy

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Electroporated intact BY-2 tobacco culture cells as a model of transient expression study.

Author(s) -

Edyta Kościańska,

Krzysztof Wypijewski

Publication year - 2001

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.2001_3900

Subject(s) - transient (computer programming) , microbiology and biotechnology , expression (computer science) , chemistry , electroporation , biology , computer science , genetics , gene , programming language , operating system

Transfer of foreign genes into plant cells can be accomplished by several methods: agrobacterium-mediated, microinjection, biolistic particle bombardment and electroporation. The last one is frequently used for transfection of plant protoplasts for transient gene expression. Electroporation is a simple procedure and allows transfecting a large number of cells at one time. Square wave-modulated porators are the most efficient for introducing expression cassettes into plant protoplasts. Based on a protocol developed by Wu & Feng (Plant Cell Reports, 1999, 18, 381-386), we optimized conditions for transfection of intact Nicotiana tabacum BY-2 cells using square wave-modulated electroporator. To simplify screening for transfected gene expression we used constructs with a GFP marker gene.

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