Evaluation of high temperature glycation of proteins and peptides by electrospray ionization mass spectrometry. | Zendy

Piotr Stefanowicz | Zendy; Janusz Boratyński | Zendy; Urszula Kańska | Zendy; Inga Petry | Zendy; Zbigniew Szewczuk | Zendy

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Evaluation of high temperature glycation of proteins and peptides by electrospray ionization mass spectrometry.

Author(s) -

Piotr Stefanowicz,

Janusz Boratyński,

Urszula Kańska,

Inga Petry,

Zbigniew Szewczuk

Publication year - 2001

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.2001_3879

Subject(s) - electrospray ionization , glycation , chemistry , mass spectrometry , protein mass spectrometry , chromatography , electrospray , extractive electrospray ionization , biochemistry , receptor

Recently Boratyński & Roy (Glycoconjugate J., 1998, 15, 131) described a fast and convenient procedure for the synthesis of glycoconjugates. In the present study we used ESI-MS and circular dichroism as tools to analyze non-enzymatic glycation products of proteins and peptides. We discuss influence of reaction conditions on the rate of glycation of lysozyme. We analyze for the first time collision induced dissociation spectra of the obtained peptide conjugates.

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