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In order to be able to study complex biological samples, a micro-capillary liquid chromatography system was coupled to a 9.4 T Fourier transform ion cyclotron resonance mass spectrometer. The setup was tested on a tryptic digest of bovine serum albumin, which resulted in high sequence coverage (> 92%) of the protein.

acta biochimica polonica

Micro-capillary liquid chromatography Fourier transform ion cyclotron resonance mass spectrometry--a powerful tool for peptide and protein identification.