Detection of damage-recognition proteins from human lymphocytes. | Zendy

Joanna Łanuszewska | Zendy; A Cudak | Zendy; Joanna RzeszowskaWolny | Zendy; Piotr Widłak | Zendy

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Detection of damage-recognition proteins from human lymphocytes.

Author(s) -

Joanna Łanuszewska,

A Cudak,

Joanna RzeszowskaWolny,

Piotr Widłak

Publication year - 2000

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.2000_4024

Subject(s) - dna , electrophoresis , microbiology and biotechnology , chemistry , dna binding protein , electrophoretic mobility shift assay , dna damage , biochemistry , biology , gene , gene expression , transcription factor

Proteins recognizing and binding to damaged DNA (DDB-proteins) were analyzed in human lymphocytes obtained from healthy donors. Using an electrophoretic mobility shift assay several complexes between nuclear extract proteins and damaged DNA were detected: a complex specific for DNA damaged by N-acetoxy-N-acetylaminofluorene, another complex specific for UV-irradiated DNA, and two complexes specific for DNA damaged by cis-dichlorodiammine platinum. All the detected complexes differed in electrophoretic mobility and possibly contained different proteins. Complexes specific for free DNA ends were also detected in protein extracts from lymphocytes.

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