Multidrug resistance-associated protein--reduction of expression in human leukaemia cells by antisense phosphorothioate olignucleotides. | Zendy

W Niewiarowski | Zendy; Edyta GendaszewskaDarmach | Zendy; Grzegorz Rębowski | Zendy; Marzena Wójcik | Zendy; Barbara Mikołajczyk | Zendy; W Goss | Zendy; Mirosław Soszyński | Zendy; Grzegorz Bartosz | Zendy

Open Access

Multidrug resistance-associated protein--reduction of expression in human leukaemia cells by antisense phosphorothioate olignucleotides.

Author(s) -

W Niewiarowski,

Edyta GendaszewskaDarmach,

Grzegorz Rębowski,

Marzena Wójcik,

Barbara Mikołajczyk,

W Goss,

Mirosław Soszyński,

Grzegorz Bartosz

Publication year - 2000

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.2000_3971

Subject(s) - hl60 , messenger rna , oligonucleotide , microbiology and biotechnology , multiple drug resistance , biology , monoclonal antibody , cell culture , chemistry , gene , cell , drug resistance , antibody , biochemistry , immunology , genetics

Multidrug resistance-associated protein (MRP1) causes cellular drug resistance in several cancer cell lines. In this paper we show that antisense oligonucleotides decrease MRP1 expression in human leukaemia cells. We investigated biological activity of a series of 12 linear phosphorothioate oligonucleotides, complementary to several regions of MRP1 mRNA. The oligonucleotides were administered to leukaemia HL60/ADR cells overexpressing MRP1 protein. Then, the level of MRP1 mRNA was determined by means of semiquantitative RT-PCR and the protein level by reaction with specific monoclonal antibodies. Some of the investigated antisense oligonucleotides decrease the expression level of the MRP1 protein by 46% and its mRNA level by 76%.

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