The DFF40/CAD endonuclease and its role in apoptosis. | Zendy

Piotr Widłak | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

The DFF40/CAD endonuclease and its role in apoptosis.

Author(s) -

Piotr Widłak

Publication year - 2000

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.2000_3957

Subject(s) - apoptotic dna fragmentation , dna fragmentation , nuclease , chromatin , icad , endonuclease , microbiology and biotechnology , fragmentation (computing) , biology , dna , apoptosis , micrococcal nuclease , biochemistry , programmed cell death , nucleosome , ecology

The sequential generation of large-scale DNA fragments followed by internucleosomal chromatin fragmentation is a biochemical hallmark of apoptosis. One of the nucleases primarily responsible for genomic DNA fragmentation during apoptosis is called DNA Fragmentation Factor 40 (DFF40) or Caspase-activated DNase (CAD). DFF40/CAD is a magnesium-dependent endonuclease specific for double stranded DNA that generates double strand breaks with 3'-hydroxyl ends. DFF40/CAD is activated by caspase-3 that cuts the nuclease's inhibitor DFF45/ICAD. The nuclease preferentially attacks chromatin in the internucleosomal linker DNA. However, the nuclease hypersensitive sites can be detected and DFF40/CAD is potentially involved in large-scale DNA fragmentation as well. DFF40/CAD-mediated DNA fragmentation triggers chromatin condensation that is another hallmark of apoptosis.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research