Energetics of Cd2+ efflux system in cadmium-resistant Staphylococcus aureus 17810R.
Author(s) -
Z Tynecka,
Anna Malm
Publication year - 1995
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.1995_4678
Subject(s) - efflux , cadmium , staphylococcus aureus , chemistry , potassium , energetics , sodium , phosphate , biophysics , membrane potential , electrochemical gradient , membrane , biochemistry , bacteria , biology , organic chemistry , ecology , genetics
Energetics of 109Cd efflux in resting cells of cadmium-resistant Staphylococcus aureus 17810R was assayed in 1 or 100 mM potassium/sodium phosphate buffer, pH7 (PB). Experiments with the use of inhibitors and ionophores showed that Cd2+ extrusion in this organism required ATP and either a pH gradient (delta pH) in 1 mM PB or membrane potential (delta psi) in 100 mM PB. The role of high phosphate ion concentration in delta psi-dependent Cd2+ efflux is discussed.
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