Synthesis, cloning and expression in Escherichia coli of the gene coding for the trypsin inhibitor from Cucurbita pepo. | Zendy

B Rempoøa | Zendy; Tadeusz Wilusz | Zendy; Wojciech T. Markiewicz | Zendy; M Fikus | Zendy

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Synthesis, cloning and expression in Escherichia coli of the gene coding for the trypsin inhibitor from Cucurbita pepo.

Author(s) -

B Rempoøa,

Tadeusz Wilusz,

Wojciech T. Markiewicz,

M Fikus

Publication year - 1995

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.1995_4676

Subject(s) - cucurbita pepo , escherichia coli , cloning (programming) , serine , gene , trypsin inhibitor , microbiology and biotechnology , gene expression , trypsin , chemistry , biochemistry , coding region , biology , molecular cloning , secretion , enzyme , botany , computer science , programming language

A chemically synthesized gene coding for the serine proteinase inhibitor CPTI II was cloned in E. coli and its expression was investigated in cytoplasmic and secretion systems. Under all conditions investigated the biologically active form of the inhibitor was found only in the latter system, although the yield was rather low.

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