Binding of Escherichia coli integration host factor (IHF) to the origin segment of p15A plasmid. | Zendy

E. Hiszczyńska-Sawicka | Zendy; Józef Kur | Zendy

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Binding of Escherichia coli integration host factor (IHF) to the origin segment of p15A plasmid.

Author(s) -

E. Hiszczyńska-Sawicka,

Józef Kur

Publication year - 1995

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.1995_4675

Subject(s) - consensus sequence , plasmid , escherichia coli , biology , footprinting , dna , binding site , bacteriophage , genetics , sequence (biology) , gene , host factor , nucleic acid sequence , sequence analysis , peptide sequence , microbiology and biotechnology , computational biology , base sequence

The integration host factor (IHF) is a sequence-specific, histone-like, multi-functional DNA-binding and -bending protein of Escherichia coli. Characterization and functional analysis of this protein has been carried out mainly in bacteriophage lambda and other mobile genetic elements. In this paper we report data concerning the binding of IHF protein to the plasmid orip15A region. IHF binds to the single site of the DNA fragment containing the orip15A, as shown by the gel mobility shift assays and footprinting experiment. On the basis of the ihf consensus sequences published, we have been able to identify one sequence of putative ihf site into the orip15A sequence with two mismatches in relation to the consensus sequence of Kur et al., 1989, Gene 81, 1-15. One ihf binding site was also found in the oriColE1 region sequence with three mismatches in relation to this consensus sequence.

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