Structural and serological characterization of Hafnia alvei lipopolysaccharide core region. | Zendy

Czesław Ługowski | Zendy; Tomasz Niedziela | Zendy; Wojciech Jachymek | Zendy; Agnieszka Klonowska | Zendy; Anna Czarny | Zendy; S Rowiński | Zendy; Carl Petersson | Zendy; Lennart Kenne | Zendy

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Structural and serological characterization of Hafnia alvei lipopolysaccharide core region.

Author(s) -

Czesław Ługowski,

Tomasz Niedziela,

Wojciech Jachymek,

Agnieszka Klonowska,

Anna Czarny,

S Rowiński,

Carl Petersson,

Lennart Kenne

Publication year - 1995

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.1995_4667

Subject(s) - hafnia , heptose , antiserum , chemistry , lipopolysaccharide , microbiology and biotechnology , epitope , serology , oligosaccharide , antigen , toxoid , biology , biochemistry , antibody , organic chemistry , immunization , immunology , cubic zirconia , ceramic , mutant , gene

The structures and serological activities of core oligosaccharide of Hafnia alvei strains have been investigated. Methylation analysis, NMR spectroscopy and various specific degradation procedures were the principal methods used. It is concluded that, core hexasaccharides are identical in the lipopolysaccharides tested and are built of two glucose, three heptose and one 2-keto-3-deoxyoctulosonic acid residues. The antiserum raised against the ATCC13337 oligosaccharide core-tetanus toxoid conjugate cross-reacted strongly with all lipopolysaccharides used as antigens in ELISA test, suggesting that this core region is the common structure in the Hafnia genus.

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