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Cloning and expression of the genes coding for tube associated proteins of bacteriophage T4.
Author(s) -
J Nieradko
Publication year - 1995
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.1995_4658
Subject(s) - gene , bacteriophage , cloning (programming) , biology , coding region , genetics , dna , molecular cloning , plasmid , microbiology and biotechnology , gene expression , escherichia coli , computer science , programming language
Genes 29, 48 and 54 of bacteriophage T4, coding for specific tube associated proteins, were cloned to the expression vector pT7-5. The molecular mass of the products of these genes was estimated to be 64, 39 and 36 kDa, respectively. The examined genes are cotranscribed with genes 51, 27 and 28 from the same DNA strand and a common late promoter sequence located downstream of gene 51.

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